As part of the creation of the UMR it was important to us to create several technical facilities that would offer high quality expertise to researchers on the PRG campus. We have dedicated a sizeable fraction of our space on the 4th floor of the Lamarck Building to house equipment that is available to researchers in the BFA, IJM and Mti units. For example, the room 413 and 425 are exclusively dedicated to equipment shared by the UMR7216 and the unit BFA. These platforms have been financed by the Direction Générale de l’Enseignement Supérieur, the CNRS, and individual grants from ARC Equipements and DIM Stem Pole. Our future plans are to increase the investment in these platforms so that the can eventually receive the national standards of Qualité to allow « Infrastructures en Biologie Santé et Agronomie » (IbiSA) labels. The motivation for these platforms was to create equipment and human resources to offer high-quality technical expertise to the PRG Life Sciences community. Our technical platforms are :
• Plateau « Epigénomique Fonctionnelle » : standardized Chromatin Immunoprecitpitation ChIP, quantitative RT-PCR, quality control, the genomic and proteomic study of histone modifications and epigenetic cellular states. (pièce 413)
• Plateau technique Vectorologie : for the production of lentiviral and retroviral vectors for infections and gene delivery in vitro and in vivo. The L3 in the Lamarck Building is the only high-security faciltiy of its kind on campus. (pièces 402 et 404, L3)
• Plateau de culture de Cellules Souches Embryonnaires (ES) murines et humaines : we now have Agence de Biomédecine authorisation for a project on X-inactivation and we are developing experimental expertise in the cultivation and genetic analysis of human ES cells. This is a powerful ressource for studying pluripotency and epigenetic regulation. We have also purchased a state-of-the-art Cell Celector for ES manipulation (pièce 452)
• Plateau « Fluorescent Microscopy » (pièces 425 et 449) : we are currently installing state-of-the-art imaging and flow cytometry for single cell analysis and the investigation of nuclear architecture.