Olivier KIRSH
Lecturer - Maître de conférence
Equipe : Dynamique de la Méthylation de l’ADN des Génomes Eucaryotes
Téléphone : +33 (0)1 57 27 89 26
Pièce n°: 438

Olivier Kirsh is Maître de Conférence (Lecturer /Associate Professor) at the Université Paris Diderot / USPC / Université de Paris.

He’s teaching biostatistics, molecular biology, functional genomic and bio-informatics from L2 to M2 at Univertsité de PARIS (Paris – Diderot, Paris 7). He’s regulary invited in panels (Magistere de Génétique, Master BI, Ecole Doctorale Compléxité du Vivant, Thèse, Comité de sélection de Maître de Conférence…). He’s also teaching for « formation continue » at the Cancéropole, L'école de bioinformatique AVIESAN de Roscoff and for the DU « Bioinformatique intégrative ».

In september 2013, Olivier joined Pierre-Antoine Defossez’s team to deploy bio-informatics and bio-analyses approaches (eg : Nanostring, ChIP-seq, RNA-seq, microarray, Whole Genome and Reduced Representation of Bisulfite Sequencing (WGBS & RRBS analyses) to study the interplay between kinases signaling pathways, chromatin remodelers and DNA methylation for the control of gene expression in cancer cell and during mouse embryonic stem cells differentiation. He’s a member of our Bioinformatic platform. He develops pipelines and scripts used to explore and analyse experiments implementing high-throughput sequencing technologies. His daily tools are R, Bash or Docker to run computational analyses on our cluster. Olivier also mentors masters and PhD students in "classical" molecular biology approaches.

Former activities:

He did his PhD at the Institut PASTEUR in Dr Anne Dejean’s lab (Unité d’Organisation Nucléaire et Oncogenèse) working on SUMO, a new Ubiquitin-like post-translational modification and senescence. He did a postdoctoral training at the Institut de Recherches Cliniques de Montréal (IRCM) in the laboratory of molecular genetics of Dr Jacques Drouin where he worked on the molecular mechanisms of nuclear receptors transcriptional repression. Then he moved to the Institut de Génétique Moléculaire de Montpellier (IGMM) in Dr Claude Sardet’s lab where he developed bio-informatics and high-throughput sequencing technologies approaches to characterized the E4F1 transcription factor target genes. His contribution enlightens unexpected functions linking cell cycle, genome integrity and metabolism for this transcription factor in cancer and post mitotics cells. He also worked with Dr Eric Julien on the H4K20me1 epigenetic mark involved in replication origins licensing.




ORCID ID : 0000-0001-6200-5681

Scopus Author ID : 6507174606


Publications :  


Genetic screens reveal mechanisms for the transcriptional regulation of tissue-specific genes in normal cells and tumors.

Naciri I, Laisné M, Ferry L, Bourmaud M, Gupta N, Dicarlo S, Huna A, Martin L, Peduto L, Bernard D, Kirsh O*, Defossez PA*.

Nucleic Acids Research, 07 February 2019 gkz080,


Mechanisms of DNA Methyltransferase Recruitment in Mammals.

Laisné M, Gupta N, Kirsh O, Pradahan S, Defossez PA.

Genes 2018, 9(12), 617; doi: 10.3390/genes9120617


The nuclear receptor RXRA controls cellular senescence by regulating calciumsignaling.
Ma X, Warnier M, Raynard C, Ferrand M, Kirsh O, Defossez PA, Martin N, Bernard D.
Aging Cell. 2018 Sep 14:e12831. doi: 10.1111/acel.12831.

PMID: 30216632.


A histone mimic within DNA Ligase 1 links DNA replication and DNA remethylation: a revised model for the maintenance of DNA methylation by UHRF1

Ferry L, Fournier A, Tsusaka T, Adelmant G, Shimazu T, Matano S, Kirsh O, Amouroux R, de Dieuleveult M, Fritsch L, Kudithipudi S, Jeltsch A, Okano M, Ait-si-ali S, Leonhardt H, Hajkova P, Marto J, Arita K, Shinkai Y, Defossez PA.

Molecular Cell, 2017, Aug 17;67(4):550-565.e5. doi: 10.1016/j.molcel.2017.07.012.

Nouvelles fonctions d’une protéine liant l’ADN méthylé dans le cancer

Naciri I, Roussel-Gervais A , Defossez PA, Kirsh O.

Médecine/Sciences (nouvelle), 2017,vol 33, numero 8-9 (aout-sept)


Loss of the methyl-CpG binding protein ZBTB4 alters the mitotic checkpoint, increases aneuploidy, and promotes tumorigenesis.

Roussel-Gervais A , Naciri I, Kirsh O, Kasprzyk L, Velasco G , Grillo G , Dubus P, Defossez PA.

Cancer Research, 2016 Nov 4. pii: canres.1181.2016


Naciri I, Defossez P.-A,Kirsh O.

Encyclopædia Universalis 2016. La science au présent, 2017

E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity

Lacroix M*, Rodier G*, Kirsh O*, Houles T, Delpech H, Seyran B, Gayte L, Casas L, Pessemesse L, Heuillet, M, Bellvert F, Portais JC, Berthet C, Bernex F, Brivet M , Boutron A ,Le Cam L and Sardet C.

PNAS, doi:10.1073/pnas.1602754113 , Sept 2016. * First co-authors


E4F1-mediated control of pyruvate dehydrogenase activity is essential for skin homeostasis.

Goguet-Rubio P*, Seyran B*, Gayte L, Bernex F, Sutter A, Delpech H, Linares LK, Riscal R, Repond C, Rodier G, Kirsh O, Touhami J, Noel J, Vincent C, Pirot N, Pavlovic G, Herault Y, Sitbon M, Pellerin L, Sardet C, Lacroix M, and Le Cam L.

PNAS doi:10.1073/pnas.1602751113, Sept 2016. * First co-authors


Screening of a kinase library reveals novel pro-senescence kinases and their common NF-κB-dependent transcriptional program.

Ferrand M, Kirsh O, Griveau A, Vindrieux D, Martin N, Defossez PA, Bernard D.

Aging (Albany NY). 2015 Nov;7(11):986-1003. PMID: 26583757


Description of an optimized ChIP-seq analysis pipeline dedicated to genome wide identification of E4F1 binding sites in primary and transformed MEFs.

Houlès T, Rodier G, Le Cam L, Sardet C, Kirsh O.

Genomics Data (2015), pp. 368-370 DOI information: 10.1016/j.gdata.2015.07.004 . PMID: 26484288


The transcription factor E4F1 coordinates CHK1-dependent checkpoint and mitochondrial functions.

Rodier G, Kirsh O*, Baraibar M, Houlès T, Lacroix M, Delpech H, Hatchi E, Arnould S, Severac D, Dubois E, Caramel J, Julien E, Friguet B, Le Cam L, Sardet C.

Cell Rep. 2015 Apr 14;11(2):220-33. doi: 10.1016/j.celrep.2015.03.024. Epub 2015 Apr 2.PMID: 25843721


E4F1 deficiency results in oxidative stress-mediated cell death of leukemic cells.

Hatchi E, Rodier G, Lacroix M, Caramel J, Kirsh O, Jacquet C, Schrepfer E, Lagarrigue S, Linares LK, Lledo G, Tondeur S, Dubus P, Sardet C, Le Cam L.

J Exp Med. 2011 Jul 4;208(7):1403-17 PMID:21708927

Coupling mitosis to DNA replication: The emerging role of the histone H4-lysine 20 methyltransferase PR-Set7.

Brustel J, Tardat M, Kirsh O, Grimaud C, Julien E.

Trends Cell Biol. 2011 Aug;21(8):452-60 PMID:21632252


The histone H4 Lys 20 methyltransferase PRSet7 regulates replication origins in mammalian cells.

Tardat M*, Brustel J*, Kirsh O, Lefevbre C, Callanan M, Sardet C, Julien E.

Nat Cell Biol. 2010 Nov;12(11):1086-93 PMID:20953199


FLI-1 functionally interacts with PIASxα, a member of the PIAS E3-SUMO-ligase family.

van den Akker, E., Ano, S., Shih, SH., Pironin, M., Palvimo, JJ., Kirsh, O., Dejean, A., Tran Quang, C., Ghysdael, J.

J Biol Chem. 2005 Nov 11;280(45):38035-46. PMID:16148010


PIAS1 interacts with the mineralocorticoid receptor via its N-terminal domain and represses its transcriptional activity- implication of SUMO-1 modification.

Pascual-le Tallec, L., Kirsh, O., Lecomte, M., Zennaro, MC., Dejean, A., Lombes, M. 

Mol Endocrinol. 2003, Dec;17(12):2529-42. PMID:14500761


The SUMO E3 ligase RanBP2 promotes modification of the HDAC4 deacetylase.

Kirsh O., Seeler, JS., Pichler, A., Gast, A., Müller, S., Miska, E., Mathieu, M., Harel-Bellan, A., Kouzarides, T., Melchior, F., Dejean, A.

EMBO J. 2002, Jun 3;21(11):2682-91. PMID:12032081

Deconstructing PML-induced premature senescence.

Bischof, O., Kirsh, O., Pearson, M., Itahana, K., Pelicci, PG., Dejean, A.

EMBO J. 2002, Jul 1;21(13):3358-69. PMID:12093737