GENIE assists researchers in experiment planning and assembly of reagents for the generation of tailor-made experimental model systems. We provide expertise and tools related to various applications of genome and epigenome engineering.
1) Maintenance of a common bank of molecular tools for genome and epigenome engineering.
To get access to the GENIE’s common bank of vectors, contact us.
2) Protocols, advice, and support for all applications of genome and epigenome engineering.
In collaboration with other local facilities, we help with custom design to fit users’ needs, generation of necessary reagents, and validation of engineered models.
GENIE assists in determining the optimal guide sequences and/or recombination template and in designing the best-suited engineering strategy for each project, with an emphasis on easy downstream screening.
GENIE also helps to choose the best way to deliver the editing components into cells. Besides the classical transfection reagents or nucleofection available in the EDC teams, full support can be provided for lentiviral delivery systems in collaboration with the PRG vectorology platform.
3) Training of new users in CRISPR-based technologies.
The course on genome editing and other CRISPR-based applications is given once a year for M2 students and other newcomers. The course can be organized on demand to small groups.
If you need to learn the basics of genome engineering and the use of CRISPR technology beyond genome and epigenome editing, contact us.
4) Testing and implementing new engineering approaches adapted to the needs of the unit.
Projects that require the development of CRISPR-based approaches, never tested previously in the unit, are chosen by the steering committee. Check our news section for the project submission deadline. To get the application form, contact us.