The microscopy facility is equipped with routine microscopes and a workstation :

– 3 widefield epifluorescence microscopes for fixed material
– 1 routine fluorescence microscope for cultured cells
– 1 widefield visible light microscope with color camera
– 1 workstation

Widefield epifluorecence microscopes (3):

Leica DMI-6000B (corner bench on the right)

Inverted epifluorescence microscope
X, Y and Z piloted
Filters and objectives piloted

Camera:
Camera CCD -30.C regulated
CoolSNAP HQ2 14bits
Image size: 1392×1040 pixels
Pixel size: 6.45 x 6.45μm

Illumination:
Visible light (halogen)
CoolLED pE-300 White Series Fluorescent Lamp

Acquisition software:
Metamorph with Multi-Dimensional Acquisition module

Objectives :

Obj Mag.	N.A	Quality	Transmission Contrast	Imm.	Pixel size (binning 1)	Max resolution at 488nm
10X	0,25	N PLAN	PH1	Air	0,645 μm	1191 nm
20X	0,35	N PLAN	PH1	Air	0,3225 μm	850 nm
40X	0,6	HCX PL Fluotar	PH2	Air	0,161 μm	496 nm
40X	1,25-0,75	HC PL APO CS	–	Oil	0,161 μm	229 nm
63X	1,40	HCX PL APO CS	PH3	Oil	0,102 μm	213 nm
100X	1,40	HCL PL APO CS	PH3	Oil	0,0645 μm	213 nm

Filters :

Filter name	Colour	Excitation Filter BP	Dichroic Mirror	Suppression Filter BP
A4	Blue	360/40	400	470/40
GFP	Green	470/40	500	525/50
Y3	Cy3 (orange)	545/40	565	610/75
TX2	Red	560/40	595	645/75
Y5	Cy5 (Far red)	620/60	660	700/75
Analyzer cube	–	–	–	Analyzer

---

Leica DMI6000B (Bench on the left, in the center)

Inverted epifluorescence microscope
Controlled in X, Y and Z
Controlled filters and lenses

Camera:
Camera CCD -12.C regulated
Teledyne Photometrics RETIGA R6
Image size: 2688×2200 pixels
Pixel size: 4.54 x 4.54μm

Illumination:
Visible light (halogen)
CoolLED pE-300 White Series Fluorescent Lamp

Acquisition software:
Metamorph with Multi-Dimensional Acquisition module

Objectives :

Mag.	N.A	Quality	Trans Contrast	Imm.	Pixel size (binning 1)	Max resolution at 488 nm
2,5X	0,07	HC FL PLAN	–	Air	1.816 μm	4,2 µm
20X	0,7	HCL PL APO CORR	–	Oil/Gly	0,227 μm	425 nm
40X	1,25-0,75	HC PL APO	–	Oil	0,1135 μm	238 nm
63X	1,40-0,6	HCX PL APO	–	Oil	0,072 μm	212 nm
100X	1,40-0,7	HCX PL APO	–	Oil	0,0454 μm	212 nm

Filters :

Name	Colour	Excitation Filter BP	Dichroic Mirror	Suppression Filter BP
XF131	Blue	387/28	410	450/65
QMAX green	Green	450-490	500	510-560
N3	Cy3 (orange)	546/12	565	600/40
TX2	Red	560/40	595	645/75
Far Red	Cy5	620-60	660	700-75
Analyzer cube	–	–	–	Analyzer

 

---

Zeiss Axioplan II (left corner bench)

Upright epifluorescence microscope
Z-controlled
Controlled filters
Manual objectives

Camera:
Camera CCD -30.C regulated
CoolSNAP fx 12bits
Image size: 1300×1030 pixels
Pixel size: 6.7 x 6.7μm

Acquisition software:
Metamorph with Multi-Dimensional Acquisition module

Illumination:
Visible light (halogen)
X-cite series 120Q fluorescent lamp

Objectives :

Mag.	N.A	Quality	Transmission Contrast	Imm.	Pixel size (binning 1)	Max resolution at 488 nm
10X	0,30	PLAN NeoFluar	–	Air	0,67 μm	992 nm
63X	1,25	PLAN NeoFluar	–	Oil	0,106 μm	238 nm
100X	1,30	PLAN NeoFluar	–	Oil	0,067μm	229 nm

Filters :

Name	Colour	Excitation Filter	Dichroic Mirror	Suppression Filter
Filter Set 01	Blue	365/12	395	LP 397
Filter Set 10	Green	450-490	510	515-565
31008 ETH BRO	Cy3 (orange)	535/35	570	605/50
Filter Set 00	Red	530-585	600	LP 615
31023 CY5	Cy5 (Far red)	640/20	660	680/30

 

Routine fluorescence microscope (1):

Leica DM IL LED (right-hand bench)

** Gloves and lab coat required on this microscope **

Inverted epifluorescence microscope

Camera:
Camera CCD -60.C regulated
Hamamatsu digital camera C4742-98-24ERG 12 or 14 bits
Image size: 1344×1024
Pixel size: 6.45 x 6.45μm

Illumination:
Visible light (halogen)
Leica fluorescent lamp

Objectives :

Mag.	N.A	Quality	Transmission Contrast	Imm.	Pixel size (binning 1)	Max resolution at 488 nm
10X	0,25	N PLAN	PH1	Air	0,645 μm	1,2 µm
20X	0,35	N PLAN	PH1	Air	0,322 μm	850 nm
40X	0,55	CORR	PH2	Air	0,161 μm	541 nm

Filters :

Name	Colour	Excitation Filter BP	Dichroic Mirror	Suppression Filter BP
B/G/R	Blue/Green/Red	420/30 ; 495 /15 ; 570/20	415 ; 510 ; 590	465/20 ; 530 /30 ; 640/40
GFP ET	Green
TX ET	Red

 

 

Microscope with color camera (1):

Leica DMRA2 (bench on the left when entering)

Upright epifluorescence microscope
Controlled in X, Y and Z

Camera:
CCD digital color camera 5Mpixels Leica DFC 450C cooled (Δ -20.C compared to ambient)
Image size: 2560×1920
Pixel size: 3.4 x 3.4μm

Illumination:
Visible light (halogen)

Objectives :

Mag.	N.A	Quality	Transmission Contrast	Imm.	Pixel size (binning 1)	Max resolution at 488 nm
10X	0,30	HC PL Fluotar	–	Air	0,34 μm	992 nm
20X	0,50	HCX PL Fluotar	–	Air	0,17 μm	595 nm
40X	0,75	HCX PL Fluotar	–	Air	0,085 μm	397 nm
100X	1,40-0,7	HCX PL APO CS	–	Oil	0,034 μm	212 nm

 

 

Workstation (1):

A workstation for image analysis is available. This workstation is equipped with the freeware FIJI, IMAGEJ, ICY, but also with Metamorph offline, Zen, and Leica Application Suite.

This workstation allows, among other things, to process 2D and 3D deconvolution images.