The microscopy facility is equipped with routine microscopes and a workstation :
– 3 widefield epifluorescence microscopes for fixed material
– 1 routine fluorescence microscope for cultured cells
– 1 widefield visible light microscope with color camera
– 1 workstation
Widefield epifluorecence microscopes (3):
Leica DMI-6000B (corner bench on the right) Inverted epifluorescence microscope Camera: Illumination: Acquisition software: Objectives : Filters : Leica DMI6000B (Bench on the left, in the center) Inverted epifluorescence microscope Camera: Illumination: Acquisition software: Objectives : Filters : Zeiss Axioplan II (left corner bench) Upright epifluorescence microscope Camera: Acquisition software: Illumination: Objectives : Filters :
X, Y and Z piloted
Filters and objectives piloted
Camera CCD -30.C regulated
CoolSNAP HQ2 14bits
Image size: 1392×1040 pixels
Pixel size: 6.45 x 6.45μm
Visible light (halogen)
CoolLED pE-300 White Series Fluorescent Lamp
Metamorph with Multi-Dimensional Acquisition moduleObj Mag. N.A Quality Transmission Contrast Imm. Pixel size (binning 1) Max resolution at 488nm 10X 0,25 N PLAN PH1 Air 0,645 μm 1191 nm 20X 0,35 N PLAN PH1 Air 0,3225 μm 850 nm 40X 0,6 HCX PL Fluotar PH2 Air 0,161 μm 496 nm 40X 1,25-0,75 HC PL APO CS – Oil 0,161 μm 229 nm 63X 1,40 HCX PL APO CS PH3 Oil 0,102 μm 213 nm 100X 1,40 HCL PL APO CS PH3 Oil 0,0645 μm 213 nm Filter name Colour Excitation Filter BP Dichroic Mirror Suppression Filter BP A4 Blue 360/40 400 470/40 GFP Green 470/40 500 525/50 Y3 Cy3 (orange) 545/40 565 610/75 TX2 Red 560/40 595 645/75 Y5 Cy5 (Far red) 620/60 660 700/75 Analyzer cube – – – Analyzer
Controlled in X, Y and Z
Controlled filters and lenses
Camera CCD -12.C regulated
Teledyne Photometrics RETIGA R6
Image size: 2688×2200 pixels
Pixel size: 4.54 x 4.54μm
Visible light (halogen)
CoolLED pE-300 White Series Fluorescent Lamp
Metamorph with Multi-Dimensional Acquisition moduleMag. N.A Quality Trans Contrast Imm. Pixel size (binning 1) Max resolution at 488 nm 2,5X 0,07 HC FL PLAN – Air 1.816 μm 4,2 µm 20X 0,7 HCL PL APO CORR – Oil/Gly 0,227 μm 425 nm 40X 1,25-0,75 HC PL APO – Oil 0,1135 μm 238 nm 63X 1,40-0,6 HCX PL APO – Oil 0,072 μm 212 nm 100X 1,40-0,7 HCX PL APO – Oil 0,0454 μm 212 nm Name Colour Excitation Filter BP Dichroic Mirror Suppression Filter BP XF131 Blue 387/28 410 450/65 QMAX green Green 450-490 500 510-560 N3 Cy3 (orange) 546/12 565 600/40 TX2 Red 560/40 595 645/75 Far Red Cy5 620-60 660 700-75 Analyzer cube – – – Analyzer
Z-controlled
Controlled filters
Manual objectives
Camera CCD -30.C regulated
CoolSNAP fx 12bits
Image size: 1300×1030 pixels
Pixel size: 6.7 x 6.7μm
Metamorph with Multi-Dimensional Acquisition module
Visible light (halogen)
X-cite series 120Q fluorescent lampMag. N.A Quality Transmission Contrast Imm. Pixel size (binning 1) Max resolution at 488 nm 10X 0,30 PLAN NeoFluar – Air 0,67 μm 992 nm 63X 1,25 PLAN NeoFluar – Oil 0,106 μm 238 nm 100X 1,30 PLAN NeoFluar – Oil 0,067μm 229 nm Name Colour Excitation Filter Dichroic Mirror Suppression Filter Filter Set 01 Blue 365/12 395 LP 397 Filter Set 10 Green 450-490 510 515-565 31008 ETH BRO Cy3 (orange) 535/35 570 605/50 Filter Set 00 Red 530-585 600 LP 615 31023 CY5 Cy5 (Far red) 640/20 660 680/30
Routine fluorescence microscope (1):
Leica DM IL LED (right-hand bench) ** Gloves and lab coat required on this microscope ** Inverted epifluorescence microscope Camera: Illumination: Objectives : Filters :
Camera CCD -60.C regulated
Hamamatsu digital camera C4742-98-24ERG 12 or 14 bits
Image size: 1344×1024
Pixel size: 6.45 x 6.45μm
Visible light (halogen)
Leica fluorescent lampMag. N.A Quality Transmission Contrast Imm. Pixel size (binning 1) Max resolution at 488 nm 10X 0,25 N PLAN PH1 Air 0,645 μm 1,2 µm 20X 0,35 N PLAN PH1 Air 0,322 μm 850 nm 40X 0,55 CORR PH2 Air 0,161 μm 541 nm Name Colour Excitation Filter BP Dichroic Mirror Suppression Filter BP B/G/R Blue/Green/Red 420/30 ; 495 /15 ; 570/20 415 ; 510 ; 590 465/20 ; 530 /30 ; 640/40 GFP ET Green TX ET Red
Microscope with color camera (1):
Leica DMRA2 (bench on the left when entering) Upright epifluorescence microscope Camera: Illumination: Objectives :
Controlled in X, Y and Z
CCD digital color camera 5Mpixels Leica DFC 450C cooled (Δ -20.C compared to ambient)
Image size: 2560×1920
Pixel size: 3.4 x 3.4μm
Visible light (halogen)Mag. N.A Quality Transmission Contrast Imm. Pixel size (binning 1) Max resolution at 488 nm 10X 0,30 HC PL Fluotar – Air 0,34 μm 992 nm 20X 0,50 HCX PL Fluotar – Air 0,17 μm 595 nm 40X 0,75 HCX PL Fluotar – Air 0,085 μm 397 nm 100X 1,40-0,7 HCX PL APO CS – Oil 0,034 μm 212 nm
Workstation (1):
A workstation for image analysis is available. This workstation is equipped with the freeware FIJI, IMAGEJ, ICY, but also with Metamorph offline, Zen, and Leica Application Suite.
This workstation allows, among other things, to process 2D and 3D deconvolution images.