Pierre-Antoine Defossez sur PUBMED

Kori S, Shibahashi Y, Ekimoto T, Nishiyama A, Yoshimi S, Yamaguchi K, Nagatoishi S, Ohta M, Tsumoto K, Nakanishi M, Defossez PA, Ikeguchi M, Arita K. Structure-based screening combined with computational and biochemical analyses identified the inhibitor targeting the binding of DNA Ligase 1 to UHRF1. Bioorg Med Chem. 2021 Dec 15;52:116500. doi: 10.1016/j.bmc.2021.116500. Epub 2021 Nov 10.

Gupta N, Yakhou L, Albert JR, Miura F, Ferry L, Kirsh O, Laisné M, Yamaguchi K, Domrane C, Bonhomme F,Sarkar A, Delagrange M, Ducos B, Greenberg MVC, Cristofari G, Bultmann S, Ito T, Defossez PA. A genome-wide knock-out screen for actors of epigenetic silencing reveals new regulators of germline genes and 2-cell like cell state. bioRxiv, 2021.2005.2003.442415.

Petryk N, Reverón-Gómez N, González-Aguilera C, Dalby M, Andersson R, Groth A. Genome-wide and sister chromatid-resolved profiling of protein occupancy in replicated chromatin with ChOR-seq and SCAR-seq. Nat Protoc. 2021 Sep;16(9):4446-4493. doi: 10.1038/s41596-021-00585-3.

Blin M, Lacroix L, Petryk N, Jaszczyszyn Y, Chen CL, Hyrien O, Le Tallec B. DNA molecular combing-based replication fork directionality profiling. Nucleic Acids Res. 2021 Jul 9;49(12):e69. doi: 10.1093/nar/gkab219.

Petryk N, Bultmann S, Bartke T, Defossez PA. Staying true to yourself: mechanisms of DNA methylation maintenance in mammals. Nucleic Acids Res. 2021 Apr 6;49(6):3020-3032. doi: 10.1093/nar/gkaa1154.

Baubec T, Defossez PA. Reading DNA Modifications. J Mol Biol. 2020 Feb 8:S0022-2836(20)30096-6. doi: 10.1016/j.jmb.2020.02.001.

Cornett EM, Ferry L, Defossez PA, Rothbart SB. Lysine Methylation Regulators Moonlighting outside the Epigenome. Mol Cell. 2019 Sep 19;75(6):1092-1101. doi: 10.1016/j.molcel.2019.08.026.

Naciri I, Laisné M, Ferry L, Bourmaud M, Gupta N, Di Carlo S, Huna A, Martin N, Peduto L, Bernard D, Kirsh O, Defossez PA. Genetic screens reveal mechanisms for the transcriptional regulation of tissue-specific genes in normal cells and tumors. Nucleic Acids Research, 07 February 2019 gkz080,

Kori S, Ferry L, Matano S, Jimenji T, Kodera N, Tsusaka T, Matsumura R, Oda T, Sato M, Dohmae N, Ando T, Shinkai Y, Defossez PA, Arita K.  Structure of the UHRF1 Tandem Tudor Domain Bound to a Methylated Non-histone Protein, LIG1, Reveals Rules for Binding and Regulation. Structure. 2018 Dec 12. pii: S0969-2126(18)30455-6. doi: 10.1016/j.str.2018.11.012.

Laisné M, Gupta N, Kirsh O, Pradahan S, Defossez PA.  « Mechanisms of DNA Methyltransferase Recruitment in Mammals ». Genes 2018, 9(12), 617; doi: 10.3390/genes9120617

Marchal C, de Dieuleveult M, Saint-Ruf C, Guinot N, Ferry L, Olalla Saad ST, Lazarini M, Defossez PA, Miotto B. Depletion of ZBTB38 potentiates the effects of DNA demethylating agents in cancer cells via CDKN1C mRNA up-regulation. Oncogenesis. 2018 Oct 11;7(10):82. doi: 10.1038/s41389-018-0092-0.

Ma X, Warnier M, Raynard C, Ferrand M, Kirsh O, Defossez PA, Martin N, Bernard D.  « The nuclear receptor RXRA controls cellular senescence by regulating calcium signaling ». Aging Cell. 2018 Sep 14:e12831. doi: 10.1111/acel.12831.

Miotto B, Marchal C, Adelmant G, Guinot N, Xie P, Marto JA, Zhang L, Defossez PA. « Stabilization of the methyl-CpG binding protein ZBTB38 by the deubiquitinase USP9X limits the occurrence and toxicity of oxidative stress in human cells. » Nucleic Acids Res. 2018 Feb 27. doi: 10.1093/nar/gky149.

Ferry L, Fournier A, Tsusaka T, Adelmant G, Shimazu T, Matano S, Kirsh O, Amouroux R, Dohmae N, Suzuki T, Filion GJ, Deng W, de Dieuleveult M, Fritsch L, Kudithipudi S, Jeltsch A, Leonhardt H, Hajkova P, Marto JA, Arita K, Shinkai Y, Defossez PA. “A histone mimic within DNA Ligase 1 links DNA replication and DNA remethylation: a revised model for the maintenance of DNA methylation by UHRF1”. Molecular Cell , 2017 Aug 17;67(4):550-565.e5. doi: 10.1016/j.molcel.2017.07.012. Epub 2017 Aug 10.

I. Naciri , A. Roussel-Gervais , P.-A. Defossez. O. Kirsh. “Nouvelles fonctions d’une protéine liant l’ADN méthylé dans le cancer”, Médecine/Sciences (nouvelle), Volume 33, Numéro 8-9, Août–Septembre 2017.

A. Roussel-Gervais , I. Naciri , O. Kirsh , L.  Kasprzyk , G. Velasco , G. Grillo , P. Dubus, P.-A. Defossez..“Loss of the methyl-CpG binding protein ZBTB4 alters the mitotic checkpoint, increases aneuploidy, and promotes tumorigenesis”, Cancer Research, 2016 Nov 4. pii: canres.1181.2016

Santolini M, Sakakibara I, Gauthier M, Ribas-Aulinas F, Takahashi H, Sawasaki T, Mouly V, Concordet JP, Defossez PA, Hakim V, Maire P. « MyoD reprogramming requires Six1 and Six4 homeoproteins: genome-wide cis-regulatory module analysis. » Nucleic Acids Res. 2016 Oct 14;44(18):8621-8640. Epub 2016 Jun 14.

M. Ferrand, O. Kirsh, A. Griveau, D. Vindrieux, N. Martin, P.-A. Defossez, and D. Bernard, “Screening of a kinase library reveals novel pro-senescence kinases and their common NF-κB-dependent transcriptional program,” Aging (Albany NY), vol. 7, no. 11, pp. 986–1003, Nov. 2015.

B. Miotto, M. Chibi, P. Xie, S. Koundrioukoff, H. Moolman-Smook, D. Pugh, M. Debatisse, F. He, L. Zhang, and P.-A. Defossez, “The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability,” Cell Rep, vol. 7, no. 2, pp. 575–587, Apr. 2014.

S. Laget, B. Miotto, H. G. Chin, P.-O. Estève, R. J. Roberts, S. Pradhan, and P.-A. Defossez, “MBD4 cooperates with DNMT1 to mediate methyl-DNA repression and protects mammalian cells from oxidative stress,” Epigenetics, vol. 9, no. 4, pp. 546–556, Apr. 2014.

H. I. Baymaz, A. Fournier, S. Laget, Z. Ji, P. W. T. C. Jansen, A. H. Smits, L. Ferry, A. Mensinga, I. Poser, A. Sharrocks, P.-A. Defossez, and M. Vermeulen, “MBD5 and MBD6 interact with the human PR-DUB complex through their methyl-CpG-binding domain,” Proteomics, vol. 14, no. 19, pp. 2179–2189, Oct. 2014.

L. Kasprzyk, P.-A. Defossez, and B. Miotto, “[Epigenetic regulation in neuronal differentiation and brain function],” Biol Aujourdhui, vol. 207, no. 1, pp. 1–17, 2013.

P.-A. Defossez, “Ceci n’est pas une DNMT: recently discovered functions of DNMT2 and their relation to methyltransferase activity (Comment on DOI 10.1002/bies.201300088),” Bioessays, vol. 35, no. 12, p. 1024, Dec. 2013.

B. A. Buck-Koehntop and P.-A. Defossez, “On how mammalian transcription factors recognize methylated DNA,” Epigenetics, vol. 8, no. 2, pp. 131–137, Feb. 2013.

K. Kim, G. Chadalapaka, S.-O. Lee, D. Yamada, X. Sastre-Garau, P.-A. Defossez, Y.-Y. Park, J.-S. Lee, and S. Safe, “Identification of oncogenic microRNA-17-92/ZBTB4/specificity protein axis in breast cancer,” Oncogene, vol. 31, no. 8, pp. 1034–1044, Feb. 2012.

A. Fournier, N. Sasai, M. Nakao, and P.-A. Defossez, “The role of methyl-binding proteins in chromatin organization and epigenome maintenance,” Brief Funct Genomics, vol. 11, no. 3, pp. 251–264, May 2012.

P.-A. Defossez and I. Stancheva, “Biological functions of methyl-CpG-binding proteins,” Prog Mol Biol Transl Sci, vol. 101, pp. 377–398, 2011.

N. Sasai, M. Nakao, and P.-A. Defossez, “Sequence-specific recognition of methylated DNA by human zinc-finger proteins,” Nucleic Acids Res., vol. 38, no. 15, pp. 5015–5022, Aug. 2010.

S. Laget, M. Joulie, F. Le Masson, N. Sasai, E. Christians, S. Pradhan, R. J. Roberts, and P.-A. Defossez, “The human proteins MBD5 and MBD6 associate with heterochromatin but they do not bind methylated DNA,” PLoS ONE, vol. 5, no. 8, p. e11982, 2010.

M. Joulie, B. Miotto, and P.-A. Defossez, “Mammalian methyl-binding proteins: what might they do?,” Bioessays, vol. 32, no. 12, pp. 1025–1032, Dec. 2010.

Q. Yu, H. Kuzmiak, Y. Zou, L. Olsen, P.-A. Defossez, and X. Bi, “Saccharomyces cerevisiae linker histone Hho1p functionally interacts with core histone H4 and negatively regulates the establishment of transcriptionally silent chromatin,” J. Biol. Chem., vol. 284, no. 2, pp. 740–750, Jan. 2009.

D. Yamada, R. Pérez-Torrado, G. Filion, M. Caly, B. Jammart, V. Devignot, N. Sasai, P. Ravassard, J. Mallet, X. Sastre-Garau, M. L. Schmitz, and P.-A. Defossez, “The human protein kinase HIPK2 phosphorylates and downregulates the methyl-binding transcription factor ZBTB4,” Oncogene, vol. 28, no. 27, pp. 2535–2544, Jul. 2009.

N. Sasai and P.-A. Defossez, “Many paths to one goal? The proteins that recognize methylated DNA in eukaryotes,” Int. J. Dev. Biol., vol. 53, no. 2–3, pp. 323–334, 2009.

A. Weber, J. Marquardt, D. Elzi, N. Forster, S. Starke, A. Glaum, D. Yamada, P.-A. Defossez, J. Delrow, R. N. Eisenman, H. Christiansen, and M. Eilers, “Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation,” EMBO J., vol. 27, no. 11, pp. 1563–1574, Jun. 2008.

E. Prokhortchouk and P.-A. Defossez, “The cell biology of DNA methylation in mammals,” Biochim. Biophys. Acta, vol. 1783, no. 11, pp. 2167–2173, Nov. 2008.

J. Nguyen, D. Yamada, D. C. Schultz, and P.-A. Defossez, “Assessment of sera for chromatin-immunoprecipitation,” BioTechniques, vol. 44, no. 1, pp. 66, 68, Jan. 2008.

S. Laget and P.-A. Defossez, “[Master and servant: epigenetic deregulations as a cause and a consequence of cancer],” Med Sci (Paris), vol. 24, no. 8–9, pp. 725–730, Sep. 2008.

M. Veron, Y. Zou, Q. Yu, X. Bi, A. Selmi, E. Gilson, and P.-A. Defossez, “Histone H1 of Saccharomyces cerevisiae inhibits transcriptional silencing,” Genetics, vol. 173, no. 2, pp. 579–587, Jun. 2006.

R. Perez-Torrado, D. Yamada, and P.-A. Defossez, “Born to bind: the BTB protein-protein interaction domain,” Bioessays, vol. 28, no. 12, pp. 1194–1202, Dec. 2006.

G. J. P. Filion, S. Zhenilo, S. Salozhin, D. Yamada, E. Prokhortchouk, and P.-A. Defossez, “A family of human zinc finger proteins that bind methylated DNA and repress transcription,” Mol. Cell. Biol., vol. 26, no. 1, pp. 169–181, Jan. 2006.

G. J. P. Filion, L. Fouvry, and P.-A. Defossez, “Using reverse electrophoretic mobility shift assay to measure and compare protein-DNA binding affinities,” Anal. Biochem., vol. 357, no. 1, pp. 156–158, Oct. 2006.

G. J. P. Filion and P.-A. Defossez, “[Epigenetics and cancer],” Bull Cancer, vol. 93, no. 4, pp. 343–347, Apr. 2006.

P.-A. Defossez, K. F. Kelly, G. J. P. Filion, R. Pérez-Torrado, F. Magdinier, H. Menoni, C. L. Nordgaard, J. M. Daniel, and E. Gilson, “The human enhancer blocker CTC-binding factor interacts with the transcription factor Kaiso,” J. Biol. Chem., vol. 280, no. 52, pp. 43017–43023, Dec. 2005.

G. Filion and P.-A. Defossez, “[Proteins binding to methylated DNA: interpreters of the epigenetic code],” Med Sci (Paris), vol. 20, no. 1, pp. 7–8, Jan. 2004.

E. Lebrun, G. Fourel, P.-A. Defossez, and E. Gilson, “A methyltransferase targeting assay reveals silencer-telomere interactions in budding yeast,” Mol. Cell. Biol., vol. 23, no. 5, pp. 1498–1508, Mar. 2003.

S.-J. Lin, M. Kaeberlein, A. A. Andalis, L. A. Sturtz, P.-A. Defossez, V. C. Culotta, G. R. Fink, and L. Guarente, “Calorie restriction extends Saccharomyces cerevisiae lifespan by increasing respiration,” Nature, vol. 418, no. 6895, pp. 344–348, Jul. 2002.

G. Fourel, T. Miyake, P.-A. Defossez, R. Li, and E. Gilson, “General regulatory factors (GRFs) as genome partitioners,” J. Biol. Chem., vol. 277, no. 44, pp. 41736–41743, Nov. 2002.

P.-A. Defossez and E. Gilson, “The vertebrate protein CTCF functions as an insulator in Saccharomyces cerevisiae,” Nucleic Acids Res., vol. 30, no. 23, pp. 5136–5141, Dec. 2002.

P. A. Defossez, S. J. Lin, and D. S. McNabb, “Sound silencing: the Sir2 protein and cellular senescence,” Bioessays, vol. 23, no. 4, pp. 327–332, Apr. 2001.

S. J. Lin, P. A. Defossez, and L. Guarente, “Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae,” Science, vol. 289, no. 5487, pp. 2126–2128, Sep. 2000.

P. U. Park, P. A. Defossez, and L. Guarente, “Effects of mutations in DNA repair genes on formation of ribosomal DNA circles and life span in Saccharomyces cerevisiae,” Mol. Cell. Biol., vol. 19, no. 5, pp. 3848–3856, May 1999.

P. A. Defossez, R. Prusty, M. Kaeberlein, S. J. Lin, P. Ferrigno, P. A. Silver, R. L. Keil, and L. Guarente, “Elimination of replication block protein Fob1 extends the life span of yeast mother cells,” Mol. Cell, vol. 3, no. 4, pp. 447–455, Apr. 1999.

P. A. Defossez, P. U. Park, and L. Guarente, “Vicious circles: a mechanism for yeast aging,” Curr. Opin. Microbiol., vol. 1, no. 6, pp. 707–711, Dec. 1998.

Y. de Launoit, J. L. Baert, A. Chotteau, D. Monte, P. A. Defossez, L. Coutte, H. Pelczar, and F. Leenders, “Structure-function relationships of the PEA3 group of Ets-related transcription factors,” Biochem. Mol. Med., vol. 61, no. 2, pp. 127–135, Aug. 1997.

P. A. Defossez, J. L. Baert, M. Monnot, and Y. de Launoit, “The ETS family member ERM contains an alpha-helical acidic activation domain that contacts TAFII60,” Nucleic Acids Res., vol. 25, no. 22, pp. 4455–4463, Nov. 1997.

A. Chotteau-Lelièvre, X. Desbiens, H. Pelczar, P. A. Defossez, and Y. de Launoit, “Differential expression patterns of the PEA3 group transcription factors through murine embryonic development,” Oncogene, vol. 15, no. 8, pp. 937–952, Aug. 1997.

J. Schneikert, H. Peterziel, P. A. Defossez, H. Klocker, Y. de Launoit, and A. C. Cato, “Androgen receptor-Ets protein interaction is a novel mechanism for steroid hormone-mediated down-modulation of matrix metalloproteinase expression,” J. Biol. Chem., vol. 271, no. 39, pp. 23907–23913, Sep. 1996.

D. Monté, L. Coutte, F. Dewitte, P. A. Defossez, M. Le Coniat, D. Stéhelin, R. Berger, and Y. de Launoit, “Genomic organization of the human ERM (ETV5) gene, a PEA3 group member of ETS transcription factors,” Genomics, vol. 35, no. 1, pp. 236–240, Jul. 1996.

M. P. Laget, P. A. Defossez, O. Albagli, J. L. Baert, F. Dewitte, D. Stehelin, and Y. de Launoit, “Two functionally distinct domains responsible for transactivation by the Ets family member ERM,” Oncogene, vol. 12, no. 6, pp. 1325–1336, Mar. 1996.

D. Monté, J. L. Baert, M. P. Laget, P. A. Defossez, L. Coutte, H. Pelczar, I. Angeli, F. Dewitte, D. Stéhelin, and Y. De Launoit, “[Transcription factors of the PEA3 group in mammary cancer],” Ann. Endocrinol. (Paris), vol. 56, no. 5, pp. 547–551, 1995.

D. Monté, J. L. Baert, P. A. Defossez, Y. de Launoit, and D. Stéhelin, “Molecular cloning and characterization of human ERM, a new member of the Ets family closely related to mouse PEA3 and ER81 transcription factors,” Oncogene, vol. 9, no. 5, pp. 1397–1406, May 1994.