High Throughput Microscopy
The platform is equipped with a PerkinElmer® Operetta CLS, which allows for microscopy screening experiments as well as automated acquisition and analysis.
The microscope has an epifluorescence or confocal mode and can be used with live cells as it is equipped with a CO2-37°C incubation chamber.
It is open to external users.
Contact the platform for more information.
© Sandra Piquet
Technical specifications :
High content automated microscope
Controlled in X, Y and Z
Controlled filters and lenses
Brightfield, Widefield and confocal (spinning disk) mode
Camera:
Andor Zyla 5,5 sCMOS 16 bit 5,5 Mpx
Image size:
2560*2160 px
Pixel size:
6.50 um x 6.50 μm
Illumination:
LED for 4 colors (370 nm, 475nm, 545 nm, 630 nm)
Objectives:
|
Mag. |
N.A |
Quality |
Transmission Contrast |
Imm. |
Pixel size (binning 1) |
Max resolution at 488 nm |
|
5X |
0,16 |
Perkin WD : 12,1 nm |
– |
Air |
1,3 μm |
2,6 μm |
|
10X |
0,3 |
Perkin WD : 5,2 nm |
– |
Air |
0,65 μm |
1,3 μm |
|
20X |
0,4 |
Perkin WD : 8,28 nm |
– |
Air |
0,325 μm |
790 nm |
|
20X water |
1 |
Perkin WD : 1,7 nm |
– |
Water |
0,325 μm |
660 nm |
|
40X water |
1,1 |
Perkin WD : 0,62 nm |
– |
Water |
0,1625 μm |
330 nm |
|
63X water |
1,15 |
Perkin WD : 0,6 nm |
– |
Water |
0,103 μm
|
280 nm |
Filters:
|
Name |
Colour |
Excitation Filter BP |
Dichroic Mirror |
Suppression Filter BP |
|
UV |
blue |
380/40 |
425 |
430-500 |
|
Green |
green |
475/30 |
495 |
500-550 |
|
Red |
red |
535/30 |
565 |
570-650 |
|
Far red |
Far red |
630/20 |
650 |
655-760 |
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